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Mechanistically, acetyl-CoA, a derivative of BCKAs, inhibits the interaction between PR domain-containing protein 16 (PRDM16) and peroxisome proliferator-activated receptor-γ (PPAR-γ) by acetylating the k915 site of PRDM16 to maintain WAT characteristics
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This multifaceted pathway, encompassing the folate and methionine cycles, enables cancer cells to synthesize and utilize single-carbon moieties for fundamental biological functions, including nucleotide biosynthesis, DNA methylation, and redox balance