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Studies on the chemical constituents of Phyllanthus emblica

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pLx-gRNA (Addgene#50662) expression plasmid encoding guide RNA was generated and co-transfected with Streptococcus pyogenes Cas9 expression plasmid and transfected into HT1080 cells in the presence of 0.2 mM Trolox using Lipofectamine 2000 (Life Technologies)