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These alterations include dysfunctional potassium and glutamate buffering, modulation of aquaporins and adenosine activity, disturbances in gap junctions, disruption of glutamate-glutamine cycle, impairment of cysteine-glutamate antiporter system and mutations in potassium channel genes (Lewerenz et al., 2013

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We observed that biobank samples (frozen within 3-17 hours) and fast-frozen in-house samples (within ∼1 hour) showed slight variations in metabolite levels