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This “endogenous, pulsatile” release pattern is the subject of research on the physiology of the GH/IGF-1 axis, the regulation of slow-wave sleep (in which the strongest nocturnal GH pulse occurs), and the differences between secretagogue stimulation and the administration of recombinant GH
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To investigate whether VGF suppression affects glucagon levels in GCGR-KO islets, we isolated islets from GCGR-KO mice and transfected them with adenovirus expressing sh-Control or sh-VGF