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LC–MS/MS Detection – Quantification occurs using targeted tandem mass spectrometry (MS/MS) with multiple reaction monitoring and stable isotope internal standards

relaxation-and-recovery-collection

PACK:Advanced - Pack of 3 (60 Tabs)

The supernatant of protein extracts was heated at 70 °C for 10 min in SDS loading buffer (2% (w/v) SDS, 10% (w/v) glycerol, 0.01% (w/v) bromophenol blue and 0.125 M Tris-HCl, 0.3 M DTT, pH 6.8) and loaded on SDS-PAGE acrylamide gels for western blotting