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Approximately 5 × 10 3 cells were seeded in each well of a 96-well plate overnight, following which the cells were applied hemin for 18 h, GHK was added to the culture medium simultaneously

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Addition of a reaction mixture converts all the GSSG into free GSH, which then reacts with the excess ThioStar ® during a second 15 minute incubation, yielding the signal related to Total GSH content

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