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Following chronic (6 week) feeding with an ethanol-containing liquid diet, the Gclm KO mice were unexpectedly found to be protected against steatosis despite showing increased oxidative stress (as reflected in elevated levels of CYP2E1 and protein carbonyls)

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10.1073/pnas.150084897 56 NorrgårdM

optimized to yield up to 10mg/250ml culture of soluble GST tagged protein