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Quantification of the western blots confirmed that all acTrxR1 variants had a statistically significant reduction in the accumulation of higher molecular weight TrxR1 complexes compared to un-modified TrxR1 at all peroxide concentrations tested for acTrxR1 K307 , from 100 to 400 µM H 2 O 2 for acTrxR1 K200 , and from 200 to 400 µM H 2 O 2 for acTrxR1 K141 (Supplementary Figure S3)

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We made 2 burr holes in the anesthetized rats (intraperitoneal (ip) injected 40 mg/kg thiopental (Rotexmedica, Germany) and 10 mg/kg diazepam (Apaurin