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Briefly, plates were coated overnight with anti-human Fc (Jackson ImmunoResearch Laboratories 109-001-008), blocked for 2 h with PBS 2% BSA at RT, and incubated overnight at 4 °C with recombinant Gp1-Fc protein derived from HEK293 cells transfected with LCMV glycoprotein vector

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S-Nitroso-L-glutathione (GSNO), Glutathione oxidized (GSSG) can be retained, separated, and analyzed using a reverse-phase Primesep 200, 3.2 x 100 mm, 5 µm, 100 A, dual ended column