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The cells were incubated at room temperature for 30 minutes in the dark, and the cell cycle was analyzed by flow cytometry (Win Bryte HS cytometer [Bio-Rad], using software Win Bryte, Bio-Rad Laboratories Inc., Hercules, CA, USA)

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Secretagogues vs direct HGH Direct HGH administration works but carries significant risks: potential pituitary suppression, increased cancer risk concerns, joint pain, water retention, and others