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R PAPS is synthesized in two steps: ATP sulfurylase condenses ATP and inorganic sulfate to form adenosine-5'-phosphosulfate (APS), then APS kinase phosphorylates APS to form PAPS (at the cost of a second ATP)

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In the control rats with a large lesion area and at the border of bone tissue and muscle, there was significant edema and no reaction of periosteum for mesenchymal stem cells (i.e., on post-operative day 2) (Figure 14)